| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.049666 |
| Chromosome: | chromosome 4 |
| Location: | 2703533 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g223400 | (1 of 11) PF13229 - Right handed beta helix region (Beta_helix) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGAGCTAGGTACGGCGCTGATGGCGCTGCTGGCGGCGCCGCTGTTCGT |
| Internal bar code: | AGAGATCTCGAGTCACGTGACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1715 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCAGGTGAACGGAAAGGA |
| Suggested primer 2: | AATAACCACATCCCCGCTGG |