| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.049666 |
| Chromosome: | chromosome 5 |
| Location: | 1507754 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g234656 | (1 of 57) 2.7.10.2 - Non-specific protein-tyrosine kinase / Cytoplasmic protein tyrosine kinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTGCTGCCTCCCACACATACCGTATGTACAGGCCCACCTTTAGCCAGAT |
| Internal bar code: | TCATAGTTGGTCCTCAACGACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3474 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGGTTATGGACGAGCTCTC |
| Suggested primer 2: | CAGGCAGCCTAGGATTCTCG |