Insertion cassette: | CIB2 |
Side of cassette: | 3' truncated? |
Strand: | - |
Strain: | CLIP2.049680 |
Chromosome: | chromosome 1 |
Location: | 3937850 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre01.g025850 | INO | (1 of 1) 1.13.99.1 - Inositol oxygenase / Myo-inositol oxygenase; myo-inositol oxygenase | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCAGCCTGTGAGCGGTCGCGGTCCCGGCATCACCGTACTGGTCAAGCC |
Internal bar code: | ATCGAGTGAGGGATTAACTGAT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 571 |
LEAP-Seq percent confirming: | 61.1111 |
LEAP-Seq n confirming: | 11 |
LEAP-Seq n nonconfirming: | 7 |
LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TCCACTCCTCCACTCTACGG |
Suggested primer 2: | TTCGTCAAGCGCCAGGTAAT |