| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.049685 |
| Chromosome: | chromosome 2 |
| Location: | 1969878 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g088050 | RPP30,XRP30 | Ribonuclease MRP subunit P30; (1 of 1) K03539 - ribonuclease P/MRP protein subunit RPP1 (RPP1, RPP30) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACTCAGTCTCATCAACGTCCAGGAGGCGTGCGCAGGATAAAACCTATCA |
| Internal bar code: | TTCCACTAGAACCTTGTGTTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1427 |
| LEAP-Seq percent confirming: | 68.75 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGAGGCGGCATGAATATCT |
| Suggested primer 2: | GTACATGCGTGATGTGTGGC |