| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.049707 |
| Chromosome: | chromosome 13 |
| Location: | 2463151 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g801530 | (1 of 6) 2.7.11.21 - Polo kinase / Polo-like kinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGTCATGCGTGTGCGACTGTGTGCGGGCGTGTTATGAGCTCACAAAGGC |
| Internal bar code: | TGGAACGGTTTAAAGGACAGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1200 |
| LEAP-Seq percent confirming: | 75.8621 |
| LEAP-Seq n confirming: | 22 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCCTCTTACCCACTCCCA |
| Suggested primer 2: | CCTGTGTCTGTCTGTGCACT |