| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.049710 |
| Chromosome: | chromosome 17 |
| Location: | 5839534 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g741450 | CPN60B1,CPN60 beta1 | (1 of 3) K04077 - chaperonin GroEL (groEL, HSPD1); Chaperonin 60B1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGCATGTGCTCTCCTATCCTTCTTCTGTCAGCAAATCAACCGCACAAAC |
| Internal bar code: | TATTTCAAACCGGTTCGATGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1185 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGGAGGGATTGAGGAGGAG |
| Suggested primer 2: | ATTCGTGCATTATGCCGTGC |