Insertion junction: CLIP2.049720_5

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.049720
Chromosome:chromosome 14
Location:4201758
Confidence (%):63
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACACGCGTAAAACCCCCCAAAACCCCTCCCAAGGTGACCACCCCCTCCA
Internal bar code:TCTCGCCGTAATGGTAATGGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:202
LEAP-Seq percent confirming:11.1111
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:ACGGGGTTTGAAGGGGTTTT
Suggested primer 2:CAAACTGCACTCCAAGCACC