Insertion junction: CLIP2.049721_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.049721
Chromosome:chromosome 10
Location:6249614
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g463300 (1 of 1) PTHR31515:SF0 - TRANSMEMBRANE PROTEIN intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AACCCTACACACACATAACTCCGTCGCGTCATTGGGCGGGCTTTCGTTTC
Internal bar code:GAGGATCGTCTGCTGTAAAGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:181
LEAP-Seq percent confirming:50.0
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:CACACACACCCAACACACAC
Suggested primer 2:TTCCAGACAGACTTTCGGCC