Insertion junction: CLIP2.049727_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.049727
Chromosome:chromosome 16
Location:2360253
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g659200 CYP36,CYP742A1 (1 of 1) 1.14.13.157 - 1,8-cineole 2-exo-monooxygenase / CYP3A4; Cytochrome P450, CYP4 superfamily intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCTGGGACGCGCCGGGTGCAACTGGTGTGCGGAGCGAACTGCTTTGCAA
Internal bar code:TGGGATATTGGTATAACATGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:155
LEAP-Seq percent confirming:11.3636
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:39
LEAP-Seq n unique pos:44

Suggested primers for confirmation by PCR

Suggested primer 1:GTCCAGGCATCGGAAGTCAA
Suggested primer 2:GCAGACGAACATCAAGGGGA