| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.049729 |
| Chromosome: | chromosome 12 |
| Location: | 6218621 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g536000 | ALA2 | (1 of 3) K14802 - phospholipid-transporting ATPase [EC:3.6.3.1] (DRS2, ATP8A); ATPase, aminophospholipid transporter | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGCACGTCCCCTCCTCCGTCTCCGAGGTGAGGAACAGCAGGTCCGCGGG |
| Internal bar code: | ACCCGTAGTGCACAGTAGGGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3015 |
| LEAP-Seq percent confirming: | 82.3529 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGCTCGCTCGACTTAAAAG |
| Suggested primer 2: | CGCCAATGATGCAGAACGAG |