| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.049737 |
| Chromosome: | chromosome 15 |
| Location: | 902600 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g640100 | (1 of 58) PTHR12393//PTHR12393:SF6 - SPHINGOMYELIN PHOSPHODIESTERASE RELATED // SPHINGOMYELIN PHOSPHODIESTERASE 2 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCACAGATCCGCCGGGTTGCCGCGAATGTTGTAGCGATACCAAAACGAC |
| Internal bar code: | CTTGTGTGTCGAGCGTGAAATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2732 |
| LEAP-Seq percent confirming: | 80.2083 |
| LEAP-Seq n confirming: | 77 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 96 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCGTCGTTTGCAGAACCTA |
| Suggested primer 2: | ACACACAGCTGACTCACTCG |