Insertion junction: CLIP2.049751_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.049751
Chromosome:chromosome 7
Location:2105257
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g326300 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGTATGACTACCGGGGTCGACGCGGCTACAAGACGCTCGGTATCGGTAGG
Internal bar code:AATATACGTATAGTTCCTACTC

Confirmation - LEAP-Seq

LEAP-Seq distance:348
LEAP-Seq percent confirming:37.5
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:TACCAATCCTTGCAACCCCC
Suggested primer 2:ACAGCCTCGCTCTCTTTTCC