Insertion junction: CLIP2.049765_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.049765
Chromosome:chromosome 8
Location:3591251
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g379550 MFT11,PHT4,PHT4-4,PHT4D Sodium-dependent phosphate transporter, major facilitator superfamily; (1 of 4) K08193 - MFS transporter, ACS family, solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), other (SLC17A) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCGGAGGGGGCCACGCCACACGTCAAGGCTCGCAGTGCCCACGGCGGGA
Internal bar code:GACCAACCACTTGTTTTTATGA

Confirmation - LEAP-Seq

LEAP-Seq distance:2225
LEAP-Seq percent confirming:94.6429
LEAP-Seq n confirming:53
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:56

Suggested primers for confirmation by PCR

Suggested primer 1:ATGTTGACGCGGTCCAGATT
Suggested primer 2:GGTTGGACCGTTTGTCTTGC