| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.049766 |
| Chromosome: | chromosome 12 |
| Location: | 9116542 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g544000 | RSP5 | (1 of 4) 1.1.1.65 - Pyridoxine 4-dehydrogenase / Pyridoxine dehydrogenase; Radial Spoke Protein 5 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTTGCCGCGGCCGACCCGCTGCCGGTCCTCAAGGCGCTGCAGAGGATGG |
| Internal bar code: | CCGGCAGATGTCATAGCTTCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 47 |
| LEAP-Seq percent confirming: | 9.09091 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAGGCAGTAGGAAGGAGGG |
| Suggested primer 2: | GGGATGACCACGTTTGAGGT |