Insertion junction: CLIP2.049784_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.049784
Chromosome:chromosome 16
Location:1691545
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g654700 PHC61 Pherophorin-chlamydomonas homolog 61; (1 of 71) PF12499 - Pherophorin (DUF3707) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGGTCAAGGTGATGGACATCTTGATGGTGGGCGCAGCGGACGAGCTCGG
Internal bar code:TAAGGGGCCCATTTCTTACATG

Confirmation - LEAP-Seq

LEAP-Seq distance:679
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:13
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:TTGTGGAAGTTGACGGGGAG
Suggested primer 2:CGAGCTTGACTCTCAGTGCA