Insertion junction: CLIP2.049801_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.049801
Chromosome:chromosome 9
Location:2441530
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g393450 FAS10,FAP233,FLA5 Flagellar Associated Protein 233; (1 of 21) PF02469 - Fasciclin domain (Fasciclin) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCGGCCCTTGACGGCCCGGTCGTAGTCGTTGGTGCCGTTGGGGAACTTG
Internal bar code:TTAAGAGCGCTGGAGCAACGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:3765
LEAP-Seq percent confirming:93.0233
LEAP-Seq n confirming:40
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:43

Suggested primers for confirmation by PCR

Suggested primer 1:GAGGTTCTGCACTGTCGTCA
Suggested primer 2:CCCTGCCGCAATGTGTTTAC