Insertion junction: CLIP2.049813_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.049813
Chromosome:chromosome 6
Location:6342561
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g293051 AMT1C,AMT3 Ammonium transporter; (1 of 8) K03320 - ammonium transporter, Amt family (amt, AMT, MEP) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCCCTTGATCCTTTTCCGTTGTGCTTTTCTAACACATGTTTCCCTTCGA
Internal bar code:TCGCGGCCCATAGGGACTTTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:387
LEAP-Seq percent confirming:2.77778
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:35
LEAP-Seq n unique pos:36

Suggested primers for confirmation by PCR

Suggested primer 1:CAACTCCCACTCCCATCCAC
Suggested primer 2:GGGAACAAGGTCAGATGGGG