| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.049814 |
| Chromosome: | chromosome 1 |
| Location: | 3722270 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g024251 | (1 of 1) PF00270//PF00271//PF04408 - DEAD/DEAH box helicase (DEAD) // Helicase conserved C-terminal domain (Helicase_C) // Helicase associated domain (HA2) (HA2) | 3'UTR_intron | |
| Cre01.g800070 | (1 of 1) PF08482 - ATP-dependent helicase C-terminal (HrpB_C) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCCCAAGCGACAAGCGCCCTCCACCACGGCCCGTCTCACTTGATGATCT |
| Internal bar code: | ACAATCAGGATCACCAAACGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 186 |
| LEAP-Seq percent confirming: | 75.0 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACGCAGGGCTTTAAGGAGA |
| Suggested primer 2: | CCTCCTGAAGTCTGTGGCAG |