| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.049816 |
| Chromosome: | chromosome 13 |
| Location: | 4447056 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g602550 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGCAGGGGCAGGTCGGGACGGTCCTTGTCTCTTCAGCACCAGCTGATA |
| Internal bar code: | CGGGTATTTAGGATCCGGCCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2431 |
| LEAP-Seq percent confirming: | 80.9524 |
| LEAP-Seq n confirming: | 51 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 63 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATGTGGGCATCACTCCAGT |
| Suggested primer 2: | CAACTGCAGGCCGATATTGC |