Insertion junction: CLIP2.049828_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.049828
Chromosome:chromosome 13
Location:2557718
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g801533 (1 of 2) 2.4.1.11 - Glycogen(starch) synthase / UDP-glucose--glycogen glucosyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGCGGTGGAGCAGCTTGCTGATGAGTGCTGATCGAGATGTCCTCTGCCC
Internal bar code:CTTAACTTCGCTTAGGGGTTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:2616
LEAP-Seq percent confirming:35.8209
LEAP-Seq n confirming:24
LEAP-Seq n nonconfirming:43
LEAP-Seq n unique pos:67

Suggested primers for confirmation by PCR

Suggested primer 1:GGGCGGGATAACTGTACGAG
Suggested primer 2:TGAAAGAGCGCAAGAGAGCA