Insertion junction: CLIP2.049842_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.049842
Chromosome:chromosome 2
Location:6042410
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g118801 GTR Glycosyl transferase; (1 of 1) 2.4.1.246 - Mannosylfructose-phosphate synthase / MFPS intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGAGGGGCCAAACTTTCGCTGGTCCTGCCACGGCCCACAGTGCACTGTAA
Internal bar code:CCGAGGGTTCTTAGTCGAAATA

Confirmation - LEAP-Seq

LEAP-Seq distance:4021
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:56
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:56

Suggested primers for confirmation by PCR

Suggested primer 1:CCTTCTGTGTTCTCACCCCC
Suggested primer 2:CAAACTCCACAGTGCAGCAC