| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.049850 |
| Chromosome: | chromosome 12 |
| Location: | 1486865 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g488000 | FFT4 | putative fructan fructosyltransferase; (1 of 2) 2.4.1.99 - Sucrose:sucrose fructosyltransferase / Sucrose:sucrose 1-fructosyltransferase | outside_mRNA |
| Cre12.g488050 | FFT5 | putative fructan fructosyltransferase; (1 of 1) 2.4.1.99//3.2.1.26 - Sucrose:sucrose fructosyltransferase / Sucrose:sucrose 1-fructosyltransferase // Beta-fructofuranosidase / Saccharase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGTTCCCGCGCAAGAATGCGGCCTCCACAAGTTCAGGACCTCAGGCCG |
| Internal bar code: | CAAATATACACGGGATATTTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5207 |
| LEAP-Seq percent confirming: | 95.4545 |
| LEAP-Seq n confirming: | 42 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 44 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAGAGGCCCGTTAGGATCT |
| Suggested primer 2: | CGTCAAAAACCTGCTGCGAA |