| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.049857 |
| Chromosome: | chromosome 17 |
| Location: | 6770088 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g746647 | PUF2 | (1 of 1) PTHR12537:SF12 - MATERNAL PROTEIN PUMILIO; Puf protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCGGAACAGTAGGGGCCAGGTACTTGTAAAAGTAACATACAAGCGTTC |
| Internal bar code: | ATCACGGTGAATCACTCATCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 808 |
| LEAP-Seq percent confirming: | 42.1053 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACTCGCCGAGATGAGTGAG |
| Suggested primer 2: | CTCGGCTGTTCTGAGCTTGA |