| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.049869 |
| Chromosome: | chromosome 7 |
| Location: | 3700853 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g337700 | PDC1 | 5'UTR_intron | |
| Cre07.g337750 | EBM5 | (1 of 3) PTHR31451//PTHR31451:SF1 - FAMILY NOT NAMED // MANNAN ENDO-1,4-BETA-MANNOSIDASE 2-RELATED; Mannan endo-1%252C4-beta-mannosidase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGCCTTCTAGTGGGACTCCACGCACCGCCTGTGCAGCTCCTTCCGCCGG |
| Internal bar code: | GGTCGGTCGGCCAGGAACGCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2898 |
| LEAP-Seq percent confirming: | 97.6744 |
| LEAP-Seq n confirming: | 42 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 43 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCTATCACAGCACTTGCCC |
| Suggested primer 2: | TGGCAGCTGGTGAGTTACTG |