| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.049907 |
| Chromosome: | chromosome 2 |
| Location: | 2449479 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g091550 | WHI,EZY18,PBF2 | (1 of 1) PTHR31745:SF1 - SINGLE-STRANDED DNA-BINDING PROTEIN WHY2, MITOCHONDRIAL; Whirly domain protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCGCTGGAGCTGGTGGCGGCGCGGTCATTGGGAGCAGCGAAGTTGGGAG |
| Internal bar code: | GATGCTAAAGAGAAACGATGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3544 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 91 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 91 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGCATGAGGGCTGCATTTA |
| Suggested primer 2: | TTAGGCTTAGGGGCAGGAGT |