| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.049908 |
| Chromosome: | chromosome 3 |
| Location: | 3290967 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g165700 | PDC3 | (1 of 1) 4.1.1.1 - Pyruvate decarboxylase / Pyruvic decarboxylase; Pyruvate decarboxylase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAAACGTCAATCCCCTTGCTCGTTGGGCTCGGGCACCTAGCCCGCACCA |
| Internal bar code: | GGCCGTCATTAAATAGGCCTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 790 |
| LEAP-Seq percent confirming: | 77.7778 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAACGGAGGATTCGCAATGC |
| Suggested primer 2: | CGAGGCATGTTACATGCACG |