Insertion junction: CLIP2.049909_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.049909
Chromosome:chromosome 16
Location:4894824
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g686950 CTF18 DNA damage repair and chromosome cohesion protein; (1 of 1) K11269 - chromosome transmission fidelity protein 18 (CTF18, CHL12) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATGTACGGCCCTGCCCCCGCGCGCCGCCTGTCTCCAGCTCGCAGCCACAG
Internal bar code:AGTCGGGACCTTGACTCTCGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:443
LEAP-Seq percent confirming:46.6667
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:CACACCTTGGCTGTGAAACG
Suggested primer 2:GCGTGGTATGTGAGGAGGAG