| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.049919 |
| Chromosome: | chromosome 16 |
| Location: | 6340546 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g675750 | (1 of 6) PTHR23257//PTHR23257:SF529 - SERINE-THREONINE PROTEIN KINASE // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCCGCGGCCCCTCCAACTCCAGTGGCACTGCAGCTTCGAACCTCCAGTA |
| Internal bar code: | ACCTGCTGCTCCCGCATAAAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3061 |
| LEAP-Seq percent confirming: | 96.4286 |
| LEAP-Seq n confirming: | 27 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTAGCATGACAGCAGCCTT |
| Suggested primer 2: | TGAACGACTGGGAAGACGTG |