| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.049936 |
| Chromosome: | chromosome 3 |
| Location: | 4444606 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g175500 | (1 of 2) K05770 - benzodiazapine receptor (BZRP) | CDS | |
| Cre03.g175550 | (1 of 38) IPR002048//IPR011992 - EF-hand domain // EF-hand domain pair | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAGGCCGGTGCACCGGCAATGAACCGCCGGGCCCGATGCATCAATGCAT |
| Internal bar code: | GTGGACTATTTGAATATACGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2451 |
| LEAP-Seq percent confirming: | 89.1304 |
| LEAP-Seq n confirming: | 41 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 46 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAGAGCCGGGAGATTGAGA |
| Suggested primer 2: | GGCCAGGGAACGAAGATGAA |