Insertion junction: CLIP2.049947_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.049947
Chromosome:chromosome 13
Location:437073
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g801516 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCTCGTGTCCACACATGCCGCGGCGCCAGCTAGCGGCGCACGTGGTGGC
Internal bar code:TATGAAGGCGGAATGGTTCCGA

Confirmation - LEAP-Seq

LEAP-Seq distance:1924
LEAP-Seq percent confirming:89.6552
LEAP-Seq n confirming:26
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:29

Suggested primers for confirmation by PCR

Suggested primer 1:GTATACAGACTGCGACGCGA
Suggested primer 2:CGTACTGGAGGTGAGCACAG