| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.049951 |
| Chromosome: | chromosome 16 |
| Location: | 6500722 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g674750 | (1 of 2) PF01857 - Retinoblastoma-associated protein B domain (RB_B) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGACCGCACTGTGCCTGCGAGAAGAAAGGCAAGATGACCGGAGTTGAAA |
| Internal bar code: | CGCAGTGGTTTTTCCTCTAAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2934 |
| LEAP-Seq percent confirming: | 86.2069 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTATGCGAAGGCTTGCAGT |
| Suggested primer 2: | CCACCTTTGGCTTTGTGCTC |