Insertion junction: CLIP2.049953_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.049953
Chromosome:chromosome 12
Location:2545139
Confidence (%):40
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGTGGGCCCCGCGCGCTGAAGTTGTGCGTGCAGGATCCACCCCCGCCAG
Internal bar code:TCGTATCGGTAGGACAGCGGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:3010
LEAP-Seq percent confirming:13.4021
LEAP-Seq n confirming:13
LEAP-Seq n nonconfirming:84
LEAP-Seq n unique pos:97

Suggested primers for confirmation by PCR

Suggested primer 1:CCCTTATCTGCATGCAACGC
Suggested primer 2:GCACTGTCTGCTGTCCTCTT