| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.049963 |
| Chromosome: | chromosome 2 |
| Location: | 3535147 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g099200 | (1 of 1) K06100 - symplekin (SYMPK) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGGATGACCTGTTGGAAGAAGAGGGCGAGGAGGGCGGCGGCGCCGGTGG |
| Internal bar code: | GAGCGTTGGAACGCTATAAGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1449 |
| LEAP-Seq percent confirming: | 77.7778 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACGAAACCTGCCACACCTC |
| Suggested primer 2: | GCCACTTCACTGAGGACCTC |