| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.049972 |
| Chromosome: | chromosome 14 |
| Location: | 2819098 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g801641 | (1 of 2) 2.1.1.282 - tRNA(Phe) 7-((3-amino-3-carboxypropyl)-4-demethylwyosine(37)-N(4))- methyltransferase / tRNA-yW synthesizing enzyme-3 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACATACCGCTCCTTCTGTCGCAGCCCCCGCCACGTCTGCCCTCGCGGCTG |
| Internal bar code: | CCTTAGGAACGGTTCACTGAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1666 |
| LEAP-Seq percent confirming: | 77.4194 |
| LEAP-Seq n confirming: | 24 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACAAGAACGTGTGCCACAG |
| Suggested primer 2: | CGTACCTGGATCTCGACACG |