Insertion junction: CLIP2.049976_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.049976
Chromosome:chromosome 14
Location:2762899
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g626400 CYP33,CYP743C1 (1 of 3) 1.14.14.1//5.3.99.5 - Unspecific monooxygenase / Xenobiotic monooxygenase // Thromboxane-A synthase / Thromboxane synthetase; Cytochrome P450, CYP197 superfamily intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCACCCAACCGCCAACCGCCAACCGCCCCCCCCCCACACACACACAGAT
Internal bar code:AGGGCGCTCAAAATAGTATATT

Confirmation - LEAP-Seq

LEAP-Seq distance:311
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:AGGAGAACAAGCACCTCAGC
Suggested primer 2:ACCCCATAACCTCTCCCCTC