Insertion junction: CLIP2.049977_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.049977
Chromosome:chromosome 3
Location:5670366
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g186550 (1 of 1) K11137 - telomere length regulation protein (TELO2, TEL2) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCCCAATCCGCCGTATAACGTCGCAACGTGACGCGTTGCAGAATGTCAG
Internal bar code:CTCATCATACAGCACCTCGCTG

Confirmation - LEAP-Seq

LEAP-Seq distance:2136
LEAP-Seq percent confirming:75.0
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:CGGTGCAGTGCGATACATTG
Suggested primer 2:CTGTAGCATACGAGCTGGGG