| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.049988 |
| Chromosome: | chromosome 12 |
| Location: | 5226223 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g527400 | PGM9 | Phosphoglycerate mutase, 2%252C3-bisphosphoglycerate-independent; (1 of 2) 5.4.2.12 - Phosphoglycerate mutase (2,3-diphosphoglycerate-independent) / Phosphoglyceromutase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTCAGGCATACCGCAAGCCGCCACCTCCGTCCGTTTGCATGTTTGGGCA |
| Internal bar code: | TGCTCTCATATGGAATTTTTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1936 |
| LEAP-Seq percent confirming: | 94.1176 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACACCATTTGCAGGCTGAC |
| Suggested primer 2: | CGAATCGGCGTTGCTAGTTG |