Insertion junction: CLIP2.049994_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.049994
Chromosome:chromosome 10
Location:3426026
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g444100 (1 of 2) PF04564//PF05049 - U-box domain (U-box) // Interferon-inducible GTPase (IIGP) (IIGP) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTGCCGGGGGCCGGCACGACCGAGTGCCCCGCCGCGCACTACTTCCGCG
Internal bar code:CCCTCAGGTCGTACTTGGTGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:2293
LEAP-Seq percent confirming:57.1429
LEAP-Seq n confirming:20
LEAP-Seq n nonconfirming:15
LEAP-Seq n unique pos:35

Suggested primers for confirmation by PCR

Suggested primer 1:CAGGGAGCCTTTAGGTGGTG
Suggested primer 2:AACTGCTCTCATGCGAACCA