Insertion junction: CLIP2.049995_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.049995
Chromosome:chromosome 12
Location:7648689
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g555550 (1 of 1) K05768 - gelsolin (GSN) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCCCAGGCCCAGGTCTGCGCCCACGCCCAACCCCACAACTGCCCACACG
Internal bar code:ACATCTATGATCACTTGGTACT

Confirmation - LEAP-Seq

LEAP-Seq distance:237
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:GAGGAGGAGGAGAGTTCGGT
Suggested primer 2:CCTAGCTCCGATGTGCTCAG