Insertion junction: CLIP2.050002_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.050002
Chromosome:chromosome 2
Location:6952338
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g389208 (1 of 2) PTHR11266//PTHR11266:SF17 - PEROXISOMAL MEMBRANE PROTEIN 2, PXMP2 MPV17 // PROTEIN MPV17 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGGAGGCCCCGGCACGCAAGCAGACCAGCTTCCCCCAGCCGCGCCGCTT
Internal bar code:GGAACAGTGCTAGTCAGTCTCT

Confirmation - LEAP-Seq

LEAP-Seq distance:3837
LEAP-Seq percent confirming:50.0
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:AGCTGTGGTACCAGAAGTGC
Suggested primer 2:CTGCCTAGCCAGACATACCG