Insertion cassette: | CIB2 |
Side of cassette: | 3' |
Strand: | - |
Strain: | CLIP2.050014 |
Chromosome: | chromosome 1 |
Location: | 7170285 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre01.g051350 | (1 of 3) PF13563 - 2'-5' RNA ligase superfamily (2_5_RNA_ligase2) | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGGCTGGTGGCTGTGCAGGCGCTTCTCGAGGCGGAATTCCCGGCCTGC |
Internal bar code: | CAGGATGGTTTGGGGATTTCCT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 3832 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 21 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGTATTTGAGTCCCCGTGCC |
Suggested primer 2: | CACGCTTCAATGCCATAGCC |