| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.050027 |
| Chromosome: | chromosome 1 |
| Location: | 2630720 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g015700 | SMM1 | (1 of 8) 3.4.24.71 - Endothelin-converting enzyme 1 / ECE-1; S-adenosyl-L-methionine-dependent methyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCTGGGAGGTGGGGCAGCAGGGCCGGCGCGAGGGGCCCCTGGCCGTGG |
| Internal bar code: | CTTGAATCGCGGTTCGCCTGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4174 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 63 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 63 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACAGAGTAGAGATCGGCC |
| Suggested primer 2: | ATGTAACCCCAACACCACCC |