| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.050032 |
| Chromosome: | chromosome 16 |
| Location: | 3051734 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g664200 | PDE33 | (1 of 20) 3.1.4.17 - 3',5'-cyclic-nucleotide phosphodiesterase / Cyclic AMP phosphodiesterase; 3'%252C5'-cyclic-nucleotide phosphodiesterase | CDS |
| Cre16.g664250 | OASTL2,ASL2 | O-acetylserine (Thiol)-lyase/cysteine synthase 2; (1 of 1) 4.2.1.22 - Cystathionine beta-synthase / Serine sulfhydrase | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGAAACAGGCTAGGCATCAAGTATTGGTTGAAGTTTGCCCAAGCGGAGGT |
| Internal bar code: | TGGGGCGTCTCCTAAGGCGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3238 |
| LEAP-Seq percent confirming: | 66.6667 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATCGCACGTTGACACCACA |
| Suggested primer 2: | AGGTCACCACAAGCGGTAAG |