Insertion junction: CLIP2.050038_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.050038
Chromosome:chromosome 10
Location:2628463
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g437050 ATP11 (1 of 1) K07555 - ATP synthase mitochondrial F1 complex assembly factor 1 (ATPeAF1, ATPAF1, ATP11); Assembly factor 1 for F1 component of mitochondrial ATP synthase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCAACCCAGCTGGGGCAGGTGGTCAAGTTGGATGAGCTGATCAAGAAGG
Internal bar code:ATAGGACGTCCTGCTCAGCTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:4871
LEAP-Seq percent confirming:98.1818
LEAP-Seq n confirming:54
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:55

Suggested primers for confirmation by PCR

Suggested primer 1:GGCATGAAGTAAAGAGCGCG
Suggested primer 2:CATGTGCCTTTGATACCGCG