Insertion junction: CLIP2.050052_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.050052
Chromosome:chromosome 11
Location:326479
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g801229 (1 of 7) IPR011335 - Restriction endonuclease type II-like 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAACCCAGCACTCGACGCCCTGCACCGGTCCGACTTGGGACCCCTACTGT
Internal bar code:TTCTGATTTAATCAGTATGGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:2301
LEAP-Seq percent confirming:97.1429
LEAP-Seq n confirming:34
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:35

Suggested primers for confirmation by PCR

Suggested primer 1:ACGGAAAGCGGCTTTTGAAC
Suggested primer 2:CTGATCACGGTGGTGGACAA