| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.050075 |
| Chromosome: | chromosome 17 |
| Location: | 3831686 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g727350 | uL23m,MRPL23 | Mitochondrial ribosomal protein L23; (1 of 1) K02892 - large subunit ribosomal protein L23 (RP-L23, MRPL23, rplW) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGTGGGCGTTTGAAGGGCAGAGCACTGAGACGGTGAGGCTAAGAGGCT |
| Internal bar code: | TGGAAAGGTGTCCCCCAATGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4548 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 81 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 81 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGAGAGCCATCAAGGTGTG |
| Suggested primer 2: | GGGTGGGCAAGCATATGAGT |