| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.050075 |
| Chromosome: | chromosome 17 |
| Location: | 3831694 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g727350 | uL23m,MRPL23 | Mitochondrial ribosomal protein L23; (1 of 1) K02892 - large subunit ribosomal protein L23 (RP-L23, MRPL23, rplW) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAAGCAAAGTTGACAACCAGGCGCCGCTCCCTCTTTCACCCCTGCCGCC |
| Internal bar code: | GAAATGTAGTAGACGGAGACCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 101 |
| LEAP-Seq percent confirming: | 40.0 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTGGGCAAGCATATGAGT |
| Suggested primer 2: | ACGAGAGCCATCAAGGTGTG |