Insertion junction: CLIP2.050104_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.050104
Chromosome:chromosome 3
Location:4304404
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g174150 (1 of 9) IPR000210//IPR011042//IPR011333 - BTB/POZ domain // Six-bladed beta-propeller, TolB-like // POZ domain 5'UTR
Cre03.g174200 (1 of 9) IPR000210//IPR011042//IPR011333 - BTB/POZ domain // Six-bladed beta-propeller, TolB-like // POZ domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTCAAGGCACCAAAAGTAGACATATCAGCGCGCTCGATCGTCGCTCTAC
Internal bar code:ATGGGTTCGTATAGGTTTTAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:2028
LEAP-Seq percent confirming:89.3939
LEAP-Seq n confirming:59
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:66

Suggested primers for confirmation by PCR

Suggested primer 1:TTGAGTCGCATCAGGGTCAC
Suggested primer 2:CGGAGGTCTGATCGGTCATG