| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.050133 |
| Chromosome: | chromosome 12 |
| Location: | 6949719 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g561350 | (1 of 1) PTHR13244//PTHR13244:SF8 - ZINC FINGER MYND DOMAIN CONTAINING PROTEIN 10 // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTCCATGCAGCTCCATGCAAGACGCGGAGCAAGGACAGCAGCAGCAGCA |
| Internal bar code: | GATAGCGAGTCCACGGGTCTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3759 |
| LEAP-Seq percent confirming: | 95.092 |
| LEAP-Seq n confirming: | 155 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 163 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGCATCTCCATCTTACCGA |
| Suggested primer 2: | GCCTACCTGTTGCTGCAGTA |