Insertion junction: CLIP2.050164_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.050164
Chromosome:chromosome 7
Location:2017546
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g325761 (1 of 2) IPR000104//IPR003439//IPR003593//IPR011527//IPR027417 - Antifreeze protein, type I // ABC transporter-like // AAA+ ATPase domain // ABC transporter type 1, transmembrane domain // P-loop containing nucleoside triphosphate hydrolase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGCGCCGCCGTGGCTTATGACCATGTGCGTCAACAACACATACAGAGGC
Internal bar code:TTCGATACGAAATGCTTTCCCA

Confirmation - LEAP-Seq

LEAP-Seq distance:967
LEAP-Seq percent confirming:11.1111
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:16
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:CCTACGGGACTTCACCAACC
Suggested primer 2:CGCCACTCCTTGACTGAGTT